Microbiological contamination in veterinary clinical-surgical environments poses a risk to both animals and healthcare professionals. The CleanVet project aims to assess the microbial load and antimicrobial resistance profile of microorganisms considered highly virulent and resistant, such as Staphylococcus aureus, Enterococcus spp., and Enterobacteriaceae, in veterinary care centers (VCCs). In each VCC, samples will be collected from different surfaces in an operating room and a veterinary consultation room, prior to use, using sterile swabs and the exposure of culture media plates to assess environmental contamination. Microbiological and molecular methods will be used to isolate and identify microorganisms, along with antimicrobial susceptibility testing (EUCAST/CLSI) to characterize their resistance profile. The results will help identify critical contamination points and support the implementation of control and biosafety measures, following a One Health approach.