The intrauterine period is a critical window for lifelong health, during which environmental stressors like pesticides may interfere with fetal development and increase disease risk. Pesticides, commonly present in food and water, have been shown to disrupt endocrine and immune systems, posing greater risks during pregnancy. Placental function depends on tryptophan (TRP) metabolism, particularly the kynurenine (KYN) pathway, which produces immunomodulatory and neuroactive compounds. Disruption of this pathway by glyphosate (GLY) and related pesticides may impair immune tolerance, placental blood flow, and fetal protection. While studies suggest that pesticides can trigger inflammation and metabolic changes in the placenta, data on its specific impact on placental TRP metabolism remain limited. This study aims to quantify TRP metabolites in the urine of pregnant women and assess KYN pathway gene expression in placental tissue samples. Additionally, exposure to GLY metabolites will be evaluated in the human placenta cell model. The findings may identify biomarkers of placenta dysfunction and provide insights into potential risks of pesticide exposure to maternal and fetal health.