This project focuses on evaluating a library of 30 chalcones and structurally related flavonoid analogs, synthesized by our collaborators at the Faculty of Pharmacy, University of Porto, for their potential as novel antimitotic and anticancer agents. Previous work demonstrated that chalcones with a 3,4,5-trimethoxyphenyl group exhibit significant antiproliferative and antimitotic activity by targeting the colchicine-binding site on tubulin, disrupting microtubule dynamics. We will conduct comprehensive biological assessments including antiproliferative assays across three cancer cell lines, live-cell imaging to monitor cell fate and apoptosis induction, tubulin polymerization inhibition assays, and western blot analyses to evaluate molecular mechanisms. Complementary functional assays will explore effects on cancer cell migration, invasion, and angiogenesis. The project aims to identify lead compounds with improved efficacy and reduced toxicity compared to current microtubule-targeting drugs. Designed for completion within one year and a modest budget, the results will pave the way for future larger-scale funding applications.